Zvaní řečníci

 


Jonathan M. Irish, Ph.D.
Assistant Professor of Cell & Developmental Biology and Pathology, Microbiology & Immunology

Dr. Jonathan Irish trained in cancer biology, immunology, and computational biology at Stanford with Garry Nolan during his Ph.D. research and Ron Levy during his postdoctoral studies.  His lab at Vanderbilt studies how signaling governs healthy cell functions and human disease outcomes.  The lab's approach focuses on single cell tools capable of targeting signaling networks in cell subsets and dissecting immune interactions of cells within human tissue biopsies obtained before and during therapy.  Dr. Irish is also the co-creator of Cytobank cloud software for single cell data analysis.  Recent advances from his group include bioinformatics tools like marker enrichment modeling (MEM), a tool for machine learning of cell identity, and application of mass cytometry (CyTOF) to reveal new, clinically significant cell subsets in human tissue and tumors.  Current projects in the Irish lab focus on cancer and immunology research.

Lab website: http://my.vanderbilt.edu/irishlab/  

Selected recent publications:
Diggins KE, Greenplate AR, Leelatian N, Wogsland CE, Irish JM. Characterizing cell subsets in heterogeneous tissues using marker enrichment modeling. Nature Methods 2017. PMC5330853. Pubmed. DOI.
Leelatian N*, Doxie DB*, Greenplate AR, Lehman JM, Sinnaeve J, Mobley BC, Kauffmann RM, Werkhaven JA, Mistry AM, Weaver KD, Thompson RC, Massion PP, Hooks MA, Kelley MC, Chambless LB, Ihrie RA, Irish JM. Single cell analysis of human tissues and solid tumors with mass cytometry. Cytometry B. PMC5459378. Pubmed. DOI.
Roussel M*, Ferrell PB, Greenplate AR, Lhomme F, Le Gallou S, Diggins KE, Johnson DB, Irish JM*.  Mass cytometry deep phenotyping of human mononuclear phagocytes and myeloid-derived suppressor cells from human blood and bone marrow. Journal of Leukocyte Biology 2017 Aug;102(2):437-447. PMID: 28400539. PMCID in progress. Pubmed. DOI.


Peter Sutovsky, PhD, Dr.h.c.
Reproductive Physiology, University of Missouri, Division of Animal Sciences and Departments of Obstetrics, Gynecology and Women’s Health, Division of Animal Sciences, Columbia


Peter Sutovsky is Professor of Animal Science in the College of Agriculture, Food and Natural Resources, University of Missouri, also appointed as Professor of Obstetrics, Gynecology and Women’s Health at the School of Medicine, University of Missouri Health System. Since the early 90’s, Peter has studied mammalian gametogenesis, fertilization, and pre-implantation embryonic development with special emphasis on the gamete and zygotic ubiquitin-proteasome system (UPS). He was the first to describe the role of UPS in the regulation of mitochondrial inheritance and introduced a novel concept of extracellular UPS, which has been validated in reproduction and outside the reproductive biology field. In particular, Peter demonstrated the importance of sperm borne proteasomes for mammalian fertilization and the role of UPS in epididymal sperm quality control, a mechanism relevant to male fertility evaluation in livestock, and the diagnosis and treatment of human male fertility. Peter’s collaborative research on the biogenesis and post-fertilization processing of sperm head perinuclear theca influenced the optimization and safeguarding of assisted reproductive technologies/therapies such as in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Other notable collaborations include the development of transgenic pig model for the study of 26S proteasome and work on rodent model of human endometriosis. Recently, the Sutovsky laboratory has also been focusing on biomarker-based flow cytometric semen analysis and semen nanopurification aimed at improving conception rates in livestock artificial insemination (AI).


As of January 2017, Peter published 169 peer reviewed articles and monograph chapters, of which 156 were cited 5,200 times in SCOPUS (h index =38), and featured on 50 journal/book/trade publication covers. He edited two books and three journal special issues. Beside other editorial board memberships, Peter served on the Board of reviewing Editors of the Biology of Reproduction, and is currently the Section Editor for Reproductive Biology in Cell & Tissue Research, Associate Editor in Systems Biology in Reproductive Medicine and Series Editor in Advances in Anatomy, Embryology and Cell Biology. He holds 13 US and foreign patents, reflecting his involvement in technology development and commercialization. He has been recognized as the inaugural recipient of the USDA-NRI Discovery Award, Big 12 Rising Star Award, and the Distinguished Researcher Award and Faculty Entrepreneur of The Year Award by The University of Missouri. Reflecting his international efforts, Peter received honorary doctoral degree, Doctor Honoris Causa from The Czech Life Sciences University in Prague, a Honorary Professorship from the Universidad Nacional Mayor de San Marcos in Lima, Peru, the oldest University in all the Americas, and was elected a honorary Visiting Professor, Professorem Hospitem of the Charles University in Prague. His work on fertilization and infertility has been funded by USDA, NIH, NIJ, Missouri Life Sciences Trust Fund and various other public and private entities. He is a frequent plenary speaker in USA and around the world, frequent NIH panel member and a senior editor in several leading journals in his field. The Sutovsky laboratory maintains active collaborations with scientists and industry partners in Argentina, Australia, Brazil, Canada, Chile, Czech Republic, Denmark, France, India, Japan, Peru, Slovakia, South Korea, Spain, United Kingdom and USA.


 

Dr. Shyamala Maheswaran
Massachusetts General Hospital, Boston, USA

Dr. Shyamala Maheswaran’s research  is focused on defining the molecular mechanisms that drive breast cancer progression and metastasis. Breast cancer, initially confined to the primary site, eventually spreads to distal sites, including lung, liver, bone and brain, by invading into the bloodstream. Upon reaching these distal sites, the tumor cells continue to grow and evolve well after removal of the primary tumor resulting in overt metastasis and disease recurrence, the leading causes of cancer-related deaths. Using cell culture and mouse models and patient derived tissues and circulating tumor cells (CTCs) enriched from breast cancer patients’ blood, Dr. Maheswaran's laboratory characterizes the contribution of oncogenic cues, tumor microenvironment-derived signals, epithelial to mesenchymal transition and tumor heterogeneity to breast cancer progression and therapeutic responses. She collaborates closely across several disciplines including Clinicians and Engineers at MGH and is currently the Scientific Director of the Center for Cancer Risk Assessment at the Massachusetts General Hospital (MGH).


SELECTED PUBLICATIONS

Comaills V, Kabeche L, Morris R, Buisson R, Yu M, Madden MW, LiCausi JA, Boukhali M, Tajima K, Pan S, Aceto N, Sil S, Zheng Y, Sundaresan T, Yae T, Jordan NV, Miyamoto DT, Ting DT, Ramaswamy S, Haas W, Zou L, Haber DA, Maheswaran S. Genomic Instability Induced by Persistent Proliferation of Cells Undergoing Epithelial-to- Mesenchymal Transition. Cell Reports 2016 (in press)

Tajima K, Yae T, Javaid S, Tam O, Comaills V, Morris R, Wittner BS, Liu M, Engstrom A, Takahashi F, Black JC, Ramaswamy S, Shioda T, Hammell M, Haber DA, Whetstine JR, Maheswaran S. SETD1A modulates cell cycle progression through a miRNA network that regulates p53 target genes. Nature Comm 2015 6:8257

Aceto N, Bardia A, Miyamoto DT, Donaldson MC, Wittner BS, Spencer JA, Yu M, Pely A, Engstrom A, Zhu H, Brannigan BW, Kapur R, Stott SL, Shioda T, Ramaswamy S, Ting DT, Lin CP, Toner M, Haber DA*, Maheswaran S*. Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis. Cell. 158(5):1110-22, 2014.

Yu M, Bardia A, Wittner BS, Stott SL, Smas ME, Ting DT, Isakoff SJ, Ciciliano JC, Wells MN, Shah AM, Concannon KF, Donaldson MC, Sequist MV, Brachtel E, Sgroi D, Baselga J, Ramaswamy S, Toner M, Haber DA, Maheswaran S. Circulating Breast Tumor Cells Exhibit Dynamic Changes in Epithelial and Mesenchymal Composition. Science. 339(6119): 580-584, 2013.

Chiba N, Comaills V, Shiotani B, Takahashi F, Shimada T, Tajima K, Winokur D, Hayashida T, Willers H, Brachtel E, Vivanco MD, Haber DA, Zou L, Maheswaran S. Homeobox B9 induces epithelial-to-mesenchymal transition-associated radioresistance by accelerating DNA damage responses. Proc Natl Acad Sci U S A. 109(8):2760-5, 2012.



Mickael BOURGE

Cytometry core facility of Imagerie-Gif,  Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France

Topics:
Isolation of organelles by flow cytometry: State of the art

Scientific interests
Having used cytometry during my PhD at University Paris XI, I have decided to specialize my scientific activities around this technology to develop methods and protocols associated to biological research issues. I am now managing the cytometry core facility of Imagerie-gif, with a major context in plant cell biology.

Favorite cytometry applications:
-    DNA content analysis (Genome size, ploidy level, cell cycle, endoreplication).
-    Monitoring of genic activity by expression of fluorescent proteins (GFP, mCherry, etc.).
-    Cell metabolic activities measurement (biosensors): Calcium, pH, membrane potential, Reactive oxygen species, etc.
-    Sorting of cells (animal, plant, yeast, bacteria) and organelles.
 

SELECTED PUBLICATIONS

Argout, X., Salse, J., Aury, J.-M., Guiltinan, M.J., Droc, G., Gouzy, J., Allegre, M., Chaparro, C., Legavre, T., Maximova, S.N., Abrouk, M., Murat, F., Fouet, O., Poulain, J., Ruiz, M., Roguet, Y., Rodier-Goud, M., Barbosa-Neto, J.F., Sabot, F., Kudrna, D., Ammiraju, J.S.S., Schuster, S.C., Carlson, J.E., Sallet, E., Schiex, T., Dievart, A., Kramer, M., Gelley, L., Shi, Z., Bérard, A., Viot, C., Boccara, M., Risterucci, A.M., Guignon, V., Sabau, X., Axtell, M.J., Ma, Z., Zhang, Y., Brown, S., Bourge, M., Golser, W., Song, X., Clement, D., Rivallan, R., Tahi, M., Akaza, J.M., Pitollat, B., Gramacho, K., D’Hont, A., Brunel, D., Infante, D., Kebe, I., Costet, P., Wing, R., McCombie, W.R., Guiderdoni, E., Quetier, F., Panaud, O., Wincker, P., Bocs, S., Lanaud, C., 2011. The genome of Theobroma cacao. Nat. Genet. 43, 101–108. doi:10.1038/ng.736

Bourbousse, C., Mestiri, I., Zabulon, G., Bourge, M., Formiggini, F., Koini, M.A., Brown, S.C., Fransz, P., Bowler, C., Barneche, F., 2015. Light signaling controls nuclear architecture reorganization during seedling establishment. Proc Natl Acad Sci U S A 112, E2836–E2844. doi:10.1073/pnas.1503512112

Bourge, M., Fort, C., Soler, M.-N., Satiat-Jeunemaître, B., Brown, S.C., 2015. A pulse-chase strategy combining click-EdU and photoconvertible fluorescent reporter: tracking Golgi protein dynamics during the cell cycle. New Phytol 205, 938–950. doi:10.1111/nph.13069

Chaintreuil, C., Gully, D., Hervouet, C., Tittabutr, P., Randriambanona, H., Brown, S.C., Lewis, G.P., Bourge, M., Cartieaux, F., Boursot, M., Ramanankierana, H., D’Hont, A., Teaumroong, N., Giraud, E., Arrighi, J.-F., 2016. The evolutionary dynamics of ancient and recent polyploidy in the African semiaquatic species of the legume genus Aeschynomene. New Phytol 211, 1077–1091. doi:10.1111/nph.13956

Guefrachi, I., Pierre, O., Timchenko, T., Alunni, B., Barrière, Q., Czernic, P., Villaécija-Aguilar, J.-A., Verly, C., Bourge, M., Fardoux, J., Mars, M., Kondorosi, E., Giraud, E., Mergaert, P., 2015. Bradyrhizobium BclA Is a Peptide Transporter Required for Bacterial Differentiation in Symbiosis with Aeschynomene Legumes. MPMI 28, 1155–1166. doi:10.1094/MPMI-04-15-0094-R

Van de Velde, W., Zehirov, G., Szatmari, A., Debreczeny, M., Ishihara, H., Kevei, Z., Farkas, A., Mikulass, K., Nagy, A., Tiricz, H., Satiat-Jeunemaître, B., Alunni, B., Bourge, M., Kucho, K., Abe, M., Kereszt, A., Maroti, G., Uchiumi, T., Kondorosi, E., Mergaert, P., 2010. Plant peptides govern terminal differentiation of bacteria in symbiosis. Science 327, 1122–1126. doi:10.1126/science.1184057

 



Giuseppe Gaipa 
Tettamanti Research Center, Pediatric Clinic of University Milano Bicocca; Laboratory of Cell and Gene Therapy Stefano Verri,  San Gerardo Hospital, Monza (MB), Italy. 

Topics:
Hematology and Cell Therapy

Giuseppe Gaipa, Ph.D., is currently Senior researcher at Tettamanti Research Center and Technical Director at Laboratory of Cell and Gene Therapy Stefano Verri of San Gerardo Hospital in Monza. He graduated in 1991 in Biological Sciences at University of Milan and in 1992 he started his research and diagnostic carrier in childhood leukemia (Istituti Clinici di Perfezionamento, Milan).  In 1995 he continued in the field of childhood leukemia at Tettamanti Research Center as responsible of the Flow Cytometry Unit. In 1998 he had a Special fellow at Department of Hematology/Oncology St. Jude Children’s Research Hospital,  Memphis, TN, USA. In 2003 he obtained the PhD degree in Pediatric Hematology-Oncology at University of Milan-Bicocca. He was President of the Italian Society of Cytometry from 2011 to 2014. Throughout his career he has centered on working with various flow cytometry based techniques (leukemia phenotyping, minimal residual disease monitoring, DNA analysis and cell cycle, phospho-flow cytometry). Since 2010 He is also active as Technical Director in development and manufacturing of cell-based medicinal products at Laboratorio Stefano Verri, San Gerardo Hospital in Monza.  Member of the AIEOP-BFM flow cytometry working group and of the EuroFlow consortium. 


SELECTED PUBLICATIONS:

Theunissen P, Mejstrikova E, Sedek L, van der Sluijs-Gelling AJ, Gaipa G, Bartels M, Sobral da Costa E, Kotrová M, Novakova M, Sonneveld E, Buracchi C, Bonaccorso P, Oliveira E, Te Marvelde JG, Szczepanski T, Lhermitte L, Hrusak O, Lecrevisse Q, Grigore GE, Froňková E, Trka J, Brüggemann M, Orfao A, van Dongen JJ, van der Velden VH; EuroFlow Consortium. Standardized flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic leukemia. Blood. 2017 Jan 19;129(3):347-357.

Gaipa G, Introna M, Golay J, Nolli ML, Vallanti G, Parati E, Giordano R, Romagnoli L, Melazzini M, Biondi A, Biagi E. Development of advanced therapies in Italy: Management models and sustainability in six Italian cell factories. Cytotherapy. 2016 Apr;18(4):481-6.

Bugarin C, Sarno J, Palmi C, Savino AM, te Kronnie G, Dworzak M, Shumich A, Buldini B, Maglia O, Sala S, Bronzini I, Bourquin JP, Mejstrikova E, Hrusak O, Luria D, Basso G, Izraeli S, Biondi A, Cazzaniga G, Gaipa G; I-BFM study group. Fine tuning of surface CRLF2 expression and its associated signaling profile in childhood B-cell precursor acute lymphoblastic leukemia. Haematologica. 2015 Jun;100(6):e229-32.

Gaipa G, Basso G, Biondi A, Campana D. Detection of minimal residual disease in pediatric acute lymphoblastic leukemia. Cytometry B Clin Cytom. 2013 Nov-Dec;84(6):359-69.

Basso G, Veltroni M, Valsecchi MG, Dworzak MN, Ratei R, Silvestri D, Benetello A, Buldini B, Maglia O, Masera G, Conter V, Arico M, Biondi A, Gaipa G. Risk of relapse of childhood acute lymphoblastic leukemia is predicted by flow cytometric measurement of residual disease on day 15 bone marrow. J Clin Oncol. 2009 Nov 1;27(31):5168-74. 




Henrik Mei
Deutsches Rheumaforschungszentrum Berlin (DRFZ), Mass Cytometry Lab, Germany

Topics:
Immune Phenotyping by Mass Cytometry

Dr. Henrik Mei is currently Scientific Head of Mass Cytometry at the DRFZ Berlin. He graduated in 2004 in Medical Biotechnology at the Technical University Berlin. He earned his doctoral degree from the Humboldt University in Berlin with his thesis on the regulation of humoral memory in man, which involved studies of human B cell differentiation and plasma cell biology at the DRFZ and the Charité Rheumatology Clinics in Berlin, Germany. Henrik continued his research on human plasma cell homeostasis which earned him awards from the German Societies of Immunology and Rheumatology. Following his interest in multidimensional flow cytometry Henrik acquired grants to conduct immune monitoring projects using mass cytometry at the Human Immune Monitoring Center (HIMC) at Stanford University (USA) with Profs. Holden Maecker, Garry Fathman and Mark Davis in 2013 - 2015. In 2015, Henrik was appointed Scientific Head of Mass Cytometry at the DRFZ Berlin, where he oversees mass cytometry activities and developments, pursues immune monitoring in chronic inflammatory diseases and projects in plasma cell biology.
Henrik coordinates the German Mass Cytometry Network.

SELECTED PUBLICATIONS [1-6]

1. Mei HE, Wirries I, Frolich D, Brisslert M, Giesecke C, Grun JR, Alexander T, Schmidt S, Luda K, Kuhl AA et al: A unique population of IgG-expressing plasma cells lacking CD19 is enriched in human bone marrow. Blood 2015, 125(11):1739-1748.

2. Mei HE, Leipold MD, Schulz AR, Chester C, Maecker HT: Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. J Immunol 2015, 194(4):2022-2031.

3. Mei HE, Leipold MD, Maecker HT: Platinum-conjugated antibodies for application in mass cytometry. Cytometry A 2016, 89(3):292-300.

4. Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A et al: Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab. Blood 2010, 116(24):5181-5190.

5. Yoshida T, Mei H, Dorner T, Hiepe F, Radbruch A, Fillatreau S, Hoyer BF: Memory B and memory plasma cells. Immunol Rev 2010, 237(1):117-139.
6. Mei HE, Yoshida T, Sime W, Hiepe F, Thiele K, Manz RA, Radbruch A, Dorner T: Blood-borne human plasma cells in steady state are derived from mucosal immune responses. Blood 2009, 113(11):2461-2469.


Yvan Saeys, Ph.D.
Data Mining and Modeling for Biomedicine Group, VIB Inflammation Research Center, Department of Applied Mathematics, Computer Science and Statistics, Ghent University, Ghent, Belgium.

Topics:
Computational Data Analysis

Yvan Saeys, PhD, is currently a Group Leader at the Flemish Institute for Biotechnology (VIB),  and an Associate Professor at Ghent University.  He obtained his M.Sc. (2000) and Ph.D. (2004) in Computer Science at Ghent University, and after spending time abroad at the University of the Basque Country (2004) and the University Claude Bernard Lyon (2008), he returned to Belgium to found the DAMBI research group (Data Mining and Modeling for Biomedicine).  His research focuses on the development and application of data mining and machine learning techniques for  biological and medical applications.  Within the field of immunology he has developed novel computational tools to analyze high-dimensional cytometry data, and is advocating the use of systems approaches to immunology.  He has over 100 publications in internationally renowned journals and conferences, and is an associate editor of Nature Scientific Reports and Information Fusion.  He was selected in 2016 as an ISAC Marylou Ingram Scholar.


SELECTED PUBLICATIONS

Saeys, Y., Van Gassen, S., Lambrecht, N. (2016) Computational flow cytometry: helping to make sense of high-dimensional immunology data. Nature Reviews Immunology (16(7):449-62)

Cannoodt, R., Saelens, W., Saeys, Y. (2016) Computational methods for trajectory inference from single cell transcriptomics.  European Journal of Immunology 46(11):2496-2506

Aghaeepour N, Chattopadhyay P, Chikina M, Dhaene T, Van Gassen S, Kursa M, Lambrecht B.N., Malek M, McLachlan G.J., Qian Y et al. Saeys Y et al..  2016.  A benchmark for evaluation of algorithms for identification of cellular correlates of clinical outcomes. Cytometry A. 89(1)

Guilliams, M., Dutertre, CA, Scott, CL, McGovern, N., Sichien, D., Chakarov, S., Van Gassen, S., Chen, J.,  Poidinger, M., De Prijck, S, Tavernier, SJ, Low, I., Irac, SE, Mattar, CN, Sumatoh, HR, Low, GHL, Chung, TJK, Chan, DKH,  Tan, KT, Hon, TLK, Fossum, E., Bogen, B., Choolani, M., Chan, JKY, Larbi, A., Luche, H., Henri, S., Saeys Y, Newell, E.W., Lambrecht, BN, Malissen, B., Ginhoux, F. (2016) Aligning dendritic cells across tissues and species through unsupervised high-dimensional analysis,  Immunity 45(3):669-84.

Van Gassen, S., Callebaut, B., Van Helden, M.J., Lambrecht, B.N., Demeester, P., Dhaene, T., Saeys, Y. (2015) FlowSOM: Using self-organizing maps for visualization and interpretation of cytometry data.  Cytometry A. 87(7):636-645

https://www.uniklinik-freiburg.de/rheuim/wissenschaft-und-forschung/ag-rizzi.html




Dr. Marta Rizzi, M.D., Ph.D.
Department of Rheumatology, University Medical Center Freiburg

Topics:
Immunology, B lymphocytes, Rheumatology, Primary Immunodeficiency


Dr. Rizzi received her Medical degree in 1999 at the University of Genoa (Italy), then performed her doctoral studies on a model of somatic cell vaccination at the University of California San Diego under the mentorship of Prof. Indiveri (Genoa, Italy) and Prof. Zanetti (UCSD). She received her PhD degree from the University of Genoa (Italy). Dr. Rizzi specialized in Allergology and Clinical Immunology from the University of Genoa (Italy). Since 2006 Dr. Rizzi has been working at the University Medical Center of Freiburg (Germany), first as a Postdoc in the Center for Chronic Immunodeficiency, and since 2014 as Group leader in the Department of Rheumatology.

Dr. Rizzi research focuses on:
- human B cell development and dysregulation in primary and secondary immunodeficiencies and in autoimmune diseases
- modulation of B cell function, development and activation
- in vitro models of human B cell development


SELECTED PUBLICATIONS

Rizzi, M., Lorenzetti, R., Fischer, K., Staniek, J., Janowska, I., Troilo, A., Strohmeier, V., Erlacher, M., Kunze, M., Bannert, B., Kyburz, D., Voll, R. E., Venhoff, N., and Thiel, J. (2016) Impact of tofacitinib treatment on human B-cells in vitro and in vivo. Journal of autoimmunity

Janda, A., Schwarz, K., van der Burg, M., Vach, W., Ijspeert, H., Lorenz, M. R., Elgizouli, M., Pieper, K., Fisch, P., Hagel, J., Lorenzetti, R., Seidl, M., Roesler, J., Hauck, F., Traggiai, E., Speckmann, C., Rensing-Ehl, A., Ehl, S., Eibel, H., and Rizzi, M. (2016) Disturbed B-lymphocytes selection in autoimmune lymphoproliferative syndrome. Blood 127(18): 2193-202

Wehr, C., Gennery, A. R., Lindemans, C., Schulz, A., Hoenig, M., Marks, R., Recher, M., Gruhn, B., Holbro, A., Heijnen, I., Meyer, D., Grigoleit, G., Einsele, H., Baumann, U., Witte, T., Sykora, K. W., Goldacker, S., Regairaz, L., Aksoylar, S., Ardeniz, O., Zecca, M., Zdziarski, P., Meyts, I., Matthes-Martin, S., Imai, K., Kamae, C., Fielding, A., Seneviratne, S., Mahlaoui, N., Slatter, M. A., Gungor, T., Arkwright, P. D., van Montfrans, J., Sullivan, K. E., Grimbacher, B., Cant, A., Peter, H. H., Finke, J., Gaspar, H. B., Warnatz, K., Rizzi, M., Inborn Errors Working Party of the European Society for, B., Marrow, T., and the European Society for, I. (2015) Multicenter experience in hematopoietic stem cell transplantation for serious complications of common variable immunodeficiency. The Journal of allergy and clinical immunology 135, 988-997             

Kraus, H., Kaiser, S., Aumann, K., Bonelt, P., Salzer, U., Vestweber, D., Erlacher, M., Kunze, M., Burger, M., Pieper, K., Sic, H., Rolink, A., Eibel, H., and Rizzi, M. (2014) A feeder-free differentiation system identifies autonomously proliferating B cell precursors in human bone marrow. Journal of immunology 192, 1044-1054        

Rizzi, M., Knoth, R., Hampe, C. S., Lorenz, P., Gougeon, M. L., Lemercier, B., Venhoff, N., Ferrera, F., Salzer, U., Thiesen, H. J., Peter, H. H., Walker, U. A., and Eibel, H. (2010) Long-lived plasma cells and memory B cells produce pathogenic anti-GAD65 autoantibodies in Stiff Person Syndrome. PloS one May 5,(5) 10838



Andy Rawstron, Ph.D.

Consultant Clinical Scientist, HMDS, St. James’s Institute of Oncology, Leeds
Honorary Visiting Professor, ECSG, Dept. of Health Sciences, University of York

Andy qualified from Edinburgh University with a BSc in Immunology in 1992. He became a Clinical Scientist at the HMDS laboratory in Leeds and completed a PhD on cellular and molecular aspects of multiple myeloma in 2002. Andy is an honorary visiting Professor with the department of Health Sciences at the University of York. He has co-authored more than 140 publications in the field of haematological maligancy diagnosis and monitoring.  

Andy's work into the understanding of CLL, both in terms of defining the nature of the precursor condition Monoclonal B-Lymphocytosis and in developing and standardizing the assessment of minimal residual disease (MRD) in CLL, has been internationally recognized. His work in coordinating a standard approach to MRD analysis in CLL has encouraged close collaboration between many of the laboratories on the cutting edge of CLL research.

Andy has a hospital service commitment to develop, select and interpret relevant tests for the diagnosis, treatment and monitoring of haematological malignancy. Within this role he has developed an award-winning service for monitoring people with MGUS, CML and CLL before and after treatment, which provides regular postal monitoring for over 2,000 people.


SELECTED PUBLICATION

Andy C Rawstron, Aloysius Ssemaganda, Ruth de Tute, Chi Doughty, Darren Newton, Anna Vardi, Paul A S Evans, Kostas Stamatopoulos, Roger G Owen, Tracy Lightfoot, Katie Wakeham, Alex Karabarinde, Gershim Asiki, Robert Newton. Monoclonal B-cell lymphocytosis in a hospital-based UK population and a rural Ugandan population: a cross-sectional study. Lancet Haematol in press. 

Rawstron AC, Fazi C, Agathangelidis A, Villamor N, Letestu R, Nomdedeu J, Palacio C, Stehlikova O, Kreuzer KA, Liptrot S, O'Brien D, de Tute RM, Marinov I, Hauwel M, Spacek M, Dobber J, Kater AP, Gambell P, Soosapilla A, Lozanski G, Brachtl G, Lin K, Boysen J, Hanson C, Jorgensen JL, Stetler-Stevenson M, Yuan C, Broome HE, Rassenti L, Craig F, Delgado J, Moreno C, Bosch F, Egle A, Doubek M, Pospisilova S, Mulligan S, Westerman D, Sanders CM, Emerson R, Robins HS, Kirsch I, Shanafelt T, Pettitt A, Kipps TJ, Wierda WG, Cymbalista F, Hallek M, Hillmen P, Montserrat E, Ghia P. A complementary role of multiparameter flow cytometry and high-throughput sequencing for minimal residual disease detection in chronic lymphocytic leukemia: an European Research Initiative on CLL study. Leukemia. 2016 Apr;30(4):929-36. doi: 10.1038/leu.2015.313. Epub 2015 Dec 7.

Rawstron AC, Gregory WM, de Tute RM, Davies FE, Bell SE, Drayson MT, Cook G, Jackson GH, Morgan GJ, Child JA, Owen RG. Minimal residual disease in myeloma by flow cytometry: independent prediction of survival benefit per log reduction. Blood. 2015 Mar 19;125(12):1932-5. doi: 10.1182/blood-2014-07-590166. Epub 2015 Feb 2.

Rawstron AC, Bennett FL, O'Connor SJ, Kwok M, Fenton JA, Plummer M, de Tute R, Owen RG, Richards SJ, Jack AS, Hillmen P. Monoclonal B-cell lymphocytosis and chronic lymphocytic leukemia. N Engl J Med. 2008 Aug 7;359(6):575-83. doi: 10.1056/NEJMoa075290.

Rawstron AC, Jones RA, Ferguson C, Hughes G, Selby P, Reid C, Dalal S, Howard M, Smith G, Hillmen P, Owen RG, Jack AS.  Outreach monitoring service for patients with indolent B-cell and plasma cell disorders: a UK experience. Br J Haematol. 2007 Dec;139(5):845-8. Epub 2007 Oct 25.



Martina Johnson Pokorná

Laboratory of Fish Genetics, IAPG AS CR, v.v.i., Liběchov, Czech Republic

The main interest of our research is evolution of sex-determining mechanisms. Particular mode of sex determination is important not only at the individual organismal level (i.e. establishment of the sex during ontogeny), but it can also influence population sex ratio and therefore total evolvability and susceptibility to extinction of the lineage in question. We are predominantly focused on reptiles (Sauropsida) as a model group for our research because in general we can find large variability in sex-determining mechanisms in this group. In some lineages sex is determined by sex chromosomes (XX/XY or ZZ/ZW) and in others by environmental factors (such as temperature). We study evolution of sex determining mechanisms and also evolution of genome organisation across lineages of reptiles and for larger comparison also in other vertebrates using various technics of classical and molecular cytogenetics including fluorescent in situ hybridization, laser microdissection or chromosome sorting on flow cytometer.


SELECTED PUBLICATION

Johnson Pokorná M
, Kratochvíl L (2014) What was the ancestral sex-determining mechanism in amniote vertebrates? Biological Reviews DOI: 10.1111/brv.12156

Koubová M, Johnson Pokorná M, Rovatsos M, Farkačová K, Altmanová M, Kratochvíl L (2014) Sex determination in madagascar geckos of the genus Paroedura (Squamata: Gekkonidae): are differentiated sex chromosomes indeed so evolutionary stable? Chromosome Research 22: 441-452

Rovatsos M, Altmanová M, Johnson Pokorná M, Kratochvíl L (2014) Novel X-linked genes revealed by qPCR in the Green Anole, Anolis carolinensis. G3: Genes Genomes Genetics 114.014084

Johnson Pokorná M, Rovatsos M, Kratochvíl L (2014) Sex chromosomes and karyotype of the (nearly) mythical creature, the Gila Monster, Heloderma suspectum (Squamata: Helodermatidae). Plos One 9 e104716

Pokorná M, Altmanová M, Kratochvíl L (2014) Multiple sex chromosomes in the light of female meiotic drive in amniote vertebrates. Chromosoe Research 22: 35-44

Pokorná M, Rens W, Rovatsos M, Kratochvíl L (2014) A ZZ/ZW sex chromosome system in the Thick-Tailed Gecko (Underwoodisaurus milii; Squamata: Gekkota: Carphodactylidae), a member of the ancient gecko lineage. Cytogenetic and Genome Research 142: 190-196

Rovatsos M, Altmanová M, Pokorná M, Kratochvíl L (2014) Conserved sex chromosomes across adaptively radiated Anolis lizards. Evolution 68: 2079-2085

Rovatsos M, Pokorná M, Altmanová M, Kratochvíl L (2014) Cretaceous park of sex determination: sex chromosomes are conserved across iguanas. Biology Letters 10: 20131093

Pokorná M, Giovannotti M, Kratochvíl L, Caputo V, Olmo E, Ferguson-Smith MA, Rens W. Conservation of chromosomes syntenic with avian autosomes in squamate reptiles revealed by comparative chromosome painting. Chromosoma 121: 409-418

Pokorná M, Kratochvíl L, Kejnovský E. Microsatellite distribution on sex chromosomes at different stages of heteromorphism and heterochromatinization in two lizard species (Squamata: Eublepharidae: Coleonyx elegans and Lacertidae: Eremias velox). BMC Genetics 12: 91

Pokorná M, Giovannotti M, Kratochvil L, Kasai F, Trifonov VA, O'Brien PCM, Caputo V, Olmo E, Ferguson-Smith MA, Rens W. Strong conservation of the bird Z chromosome in reptilian genomes is revealed by comparative painting despite 275 My divergence. Chromosoma 120: 455-468

Pokorná M, Rábová M, Ráb P,  Ferguson-Smith MA, Rens W, Kratochvíl L. Differentiation of sex chromosomes and karyotypic evolution in the eye-lid geckos (Squamata: Gekkota: Eublepharidae), a group with different modes of sex determination. Chromosome Research 18: 809–820

Pokorná M, Kratochvíl L. Phylogeny of sex-determining mechanisms in squamate reptiles: Are sex chromosomes an evolutionary trap? Zoological Journal of the Linnean Society 156: 168-183